Effective murolytic solubilization of streptococcal-group-specific antigen.
نویسندگان
چکیده
Streptococcal-group-specific antigens were solubilized with a murolytic enzyme contained in the culture supernatant of Streptomyces globisporus. This facilitated the effective serogrouping of streptococci from humans and animals.
منابع مشابه
جستجوی آنتی ژن پلی ساکاریدی استر پتوکک گروه آ در مایع مفصلی بیماران مبتلا به آرتریت
Background: Diagnosing the etiologic causes for septic arthritis is very important. The main goal of study was to determine the group A streptococcal polysaccharide antigens in synovial fluid of patients with arthritis. Methods: A cross sectional study was performed upon 52 cases with acute mono arthritis in Hazrat-e-Rasool hospital in Tehran Iran (2010-2012). Techniques used were: Gr...
متن کاملجستجوی آنتی ژن پلی ساکاریدی استر پتوکک گروه آ در مایع مفصلی بیماران مبتلا به آرتریت
Background: Determining the etiologic agents of septic arthritis is very important. The aim of the present study was to determine group A streptococcal polysaccharide antigens in synovial fluid of patients with arthritis. Methods: A cross sectional study was conducted upon 52 cases with acute mono arthritis in Hazrat-e-Rasool Akram hospital in Tehran, Iran (2010-2012).Gram staining, ...
متن کاملAssay of antibody to group A streptococcal carbohydrate by enzyme-linked immunosorbent assay.
An indirect enzyme-linked immunosorbent assay system for determination of antibody levels to the group A streptococcal cell wall carbohydrate antigen is described. Optimal conditions for antigen preparation, purification, and conjugation to poly-L-lysine for adequate adsorption to the solid phase are presented. Antibody titers of unknown sera were determined by comparison to known reference sta...
متن کاملIdentification of group B streptococcal antigen with lectin-bound polystyrene particles.
The lectin of the tomato, Lycopersicon esculentum, or of the potato, Solanum tuberosum, can be passively coupled to amide-modified polystyrene spheres to be used as a detection reagent for the specific identification of group B streptococcal cultures grown in selective or nonselective Todd-Hewitt broth for 5 and 4 h, respectively. Agglutination occurred when the lectin reagents were allowed to ...
متن کاملStreptococcal M Antigen Location and Synthesis, Studied by Immunofluorescence
Streptococcal M protein has been studied directly in the intact streptococcal cell by specific immunofluorescence. By this method, it can be seen to be concentrated in or on the cell wall, but cannot be detected in the capsule. The lack of type-specific (but not group-specific) immunofluorescence after trypsinization; and the inhibition of group-specific immunofluorescence by unlabeled type-spe...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 24 5 شماره
صفحات -
تاریخ انتشار 1986